What is the excitation and emission maxima of EGFP?
As demonstrated in Figure 1, the red-shifted variants, typified by EGFP, have a single excitation peak centered at about 488 nm, with an emission peak wavelength of 509 nm.
What is the EGFP gene?
Abstract. Reporter gene fusions based on the enhanced green fluorescent protein (EGFP) are powerful experimental tools that allow real-time changes in gene expression to be monitored both in single cells and in populations.
What wavelength of light excites GFP?
GFP can be excited by the 488 nm laser line and is optimally detected at 510 nm.
What is EGFP used for?
EGFP (Enhanced green fluorescent protein) is used as a reporter protein to monitor the anchor peptide binding on polymer surfaces.
What is ZsGreen?
ZsGreen1 is a human codon-optimized variant of the Zoanthus sp. green fluorescent protein, ZsGreen, which has been engineered for brighter fluorescence and higher expression in mammalian cells. The unmodified vector will express ZsGreen1 in mammalian cells.
What is the molecular weight of EGFP?
32.7 kDa
Product Details
| abID | ab51992 |
|---|---|
| Appearance | Lyophilized protein |
| Physical Form Description | Freeze Dried |
| Molecular Weight | 32.7 kDa |
| Purity by SDS-PAGE | ≥97% |
What is EGFP expression?
Enhanced green fluorescent protein (EGFP) is a widely used biological reporter. However, the effects of EGFP expression in vivo are still unclear. To investigate the effects of EGFP transgenic expression in vivo, we employed an NMR-based metabonomics method to analyze the metabonome of EGFP transgenic mice.
How long is EGFP gene?
| Protein | Similar proteins | Length |
|---|---|---|
| C5MKY7 | EGFP | 239 |
| Enhanced green fluorescence protein | 239 | |
| Enhanced green fluorescent protein | 239 | |
| Enhanced green fluorescent protein | 239 |